International Journal of Multidisciplinary Health Sciences and Research

Category: Original Article,      DOI: Recived: 22/09/2024, Accepted: 20/09/2024, Published online: 28/10/2024

Production of ESBL and MBL in Clinical Klebsiella Isolates: A Cross-Sectional Study

Pratima Kumari1 , Saumya Singh2 , Nandlal Kumar3*

Abstract: Background: Klebsiella species are commonly detected in clinical samples and the synthesis of Metallo Beta-Lactamases (MBLs) and Extended Spectrum Beta-Lactamases (ESBLs) is a common type of drug resistance. Thus, the purpose of our investigation was to evaluate the production rate of ESBL and MBL in Klebsiella species that were isolated from the clinical specimens. Materials and Methods: Standard methods were used to isolate and identify Klebsiella species, and clinical laboratory standard recommendations were followed for antibiotic sensitivity testing, ESBL and MBL detection, and other procedures. Results: A total of 194 isolates of Klebsiella species were included in this study. Male patients were 60.82% and female patients 39.8%. The highest strains were from sputum 35.05% followed by urine 20.62%, ET-secretion 14.43%, pus 12.37%, and least from wound swab 2.06. The highest ESBL and MBL producer was from sputum 34.15%, 38.24% followed by urine 19.51%, 17.65%, ET-secretion 14.63%, 13.24% and the least was wound swab 1.22%, 1.47% respectively. Nitrofurantoin was the least resistant. Conclusion: The higher frequency of ESBL generation in isolates from inpatients raises serious concerns since it suggests that ESBL-producing bacteria are widely distributed in the population. Monitoring the rates of multidrug resistance and ESBL and MBL generation in these clinical isolates regularly is crucial.